![]() Wash with Capto DEAE At least 10 CV start buffer or until eluent pH and conductivity have reached the required values. Wash with at least 2 CV ultra pure water.ĥ. Wash with at least 2 column volumes (CV) of 2.0 M NaCl.Ĥ. Stable to commonly used aqueous buffers, 1.0 M NaOH 8 M Urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.ġ. To optimize the AEX step, a DoE was used to assess the effect of virus load, elution buffer pH and NaCl concentration for elution on LV recovery from Mustang Q and Sartobind Q membranes. DNase treatment is usually performed to further reduce. The SEC is widely used for virus purification 36. ![]() This prevents clogging and increases the life time of the column when loading large sample volumes. Because they use the same flow rate and operation buffer, the Capto Q and Capto core columns could be operated and connected via a serial connection to reduce the process time and buffer consumption required for column preparation 27. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.Ģ. HiTrap SP HP Cation exchange column: 9.2.1.1.Buffer A 0.2M sodium acetate, pH 5.0, 0.01 Tween 80 9.2.1.2.Buffer B: 0.2M sodium acetate, pH 5.0, 0. Examples of buffers for specific applications where t-PA is the protein/biopharmaceutical of interest are : 9.2.1. at least 700 cm/h in a 1 m diameter column with 20 cm bed height at 20 ☌ using process buffers with thesame viscosity as water at < 3 bar (0. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Buffer preparation is beyond the scope of this document. Arrow Quiver is Designed to Keep Your Arrows Organized and Within Easy Reach. These columns are easily connected in series to increase bed height to 20 cm.įast results and minimal consumption of sample and buffer consumption through the small bed volume.īioProcess columns packed with the same chromatography resin and using the same linear fluid velocity can produce scalable, reproducible results.ġ. ![]() Prepacked with Capto Q strong anion exchange chromatography resin.Ĭonvenient method optimization and parameter screening because of the 10cm bed height. Stable to commonly used aqueous buffers, 1.0 M NaOH 8 M Urea, 6 M guanidine hydrochloride, 30 isopropanol, and 70 ethanol. HiScreen Capto Q is a column packed with a strong anion exchange modern resin, combining speed and capacity. select an anion exchanger (Q, DEAE, ANX) with a buffer pH above the pI. ![]()
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